Quorum Sensing And Its Importance To Biotechnology

Quorum Sensing And Its Importance To Biotechnology Quorum sensing offers potential to create engineered bacteria capable of invading cancer cells. It is possible to envision the creation of novel anti-cancer therapeutics by the addition of cancer-destructing modules to these microbial biosensors. Another application of QS and quorum quenching lies in the creation of transgenic plants that are able to defend themselves against common bacterial pathogens. It plays a main role in controlling a diversity of microbial cell activities, such as biofilm formation and virulence that considerably impact human health, agriculture, and commercial production and transport systems. Quorum sensing is cell-to-cell communication in bacteria have ability to control development, sporulation, and antibiotic synthesis also virulence factor induction, cell differentiation, moreover nutrient flux along with extra physiological events in pathogenic bacterial infections. Scientists now a days creating more possible benefits from quorum sensing and off course there is lots of potential development for advancement in 2011ranging from marine to human disorders. CHAPTER 1 INTRODUCTION Several unicellular microorganisms use smallsignaling molecules to find out their local concentration. The processes involved in the production and recognitionof these signals are generally known as quorum sensing (QS). Unicellular microorganisms to manage their activities use this kind of cell-to-cell communication, which allows them to work as multi-cellular systems. Newly, several groups have confirmed artificial intraspeciesand inter-species communication through synthetic circuits, which incorporate components of bacterial QSsystems. Engineered QS-based circuits have a broad range of applications such as production of biochemicals, tissueengineering, also mixed-species fermentations. They are also extremely useful in designing microbial biosensors toidentify bacterial species present in the environment andinside living organisms. In this articlethe different ways inwhich researchers have designed QS-based circuits andtheir applications in biotechnology are explained. A decade ago, the secretion and perception of minorsignalling molecules that in turn are transduced tocoordinate behavior of a smallest unit of microorganisms was named quorum sensing by EP Greenberg with colleagues. Ever since then, an exponential growth in understanding and occurrence of quorum-sensing systemshas developed, with sightings ranging from virulence inhuman along with plant pathogens to degradative capacity ofactivated sludge. Not amazingly, regulatory mechanisms span traditional inducer/repressor motifs homologous to thelac operon to the newly discovered interfering RNAs.Advance characterization of signalling circuits, coupled with creative position applications, propose a wealth of prospects for advancing commercial biotechnology(reviewed by John C March and William E Bentley 2004). Researchers in biotechnology continuously seek new platforms from which to address problems: manifesto that, in a broad sense, improve efficacy, while maintaining or intensifying specificity. Most freshly, microbial quorum sensing has emerged as such a technology. Because microbial communities absorb a small space, concentrations of extracellular signalling molecules build up, providing motivation for unique and various cellular responses along with protection from rival microbial communities. Referred to as quorum sensing for its regularly reported and concurrent dependence on high population density (Joyce EA et al 2004), extracellular signalinggives a novel basis for control over molecular also cellular processes along with population behaviour, possibly in a manner more reliable with that of native physiology. Quorum sensing might be the base upon which the more complicated intracellular communication found in advanced level organisms has evolved. Defining quorum sensing Quorum Sensing considered a signalling molecule, a compound has to result a reaction in a population of cells that is different from the approach in which the cells would perform independently. There are two types of quorum sensing: species- specific and interspecies. Species-specific quorum sensing in Gram-negative bacteria is intercede by acyl-homoserine lactones (AHLs) with numerous moieties distinguishing signals between species (Fuqua C, Parsek MR, Greenberg EP 2001). In Gram- positive bacteria, species-specific quorum sensing is generallyassist through small peptides Figure1.Structure of bacterial Quurom sensing signals. Gram-negative bacteria like V.Fischeri and Pseudomonas aeruginosa use acyl homoserine lactones (AHLs) as signals. The structure of mature AIP-I (from Staphylococcus aureus) is shown as a representative of the translationally derived auto-inducing peptides (AIPs) used by Gram-positive bacteria as signals for QS.Source- Functions of quorum sensing Quorum sensing is supposed to control ability development, sporulation, and antibiotic synthesis also virulence factor induction, cell differentiation, and nutrient flux along with extra physiological events in pathogenic bacterial infections (Cvitkovitch DGGreenberg EP,Yarwood JM,2003).More lately, quorum sensing was connected through proteomic analysis to increased pathogenic ability in tubercular strains of Pseudomonas aeruginosa (Arevalo-Ferro C, et all 2004) Webb and co-workers (Webb JS, et al 2003), reviewed work on programmed cell death plusmicro colony differentiation in biofilms. As biofilms age, cellular differentiation and death improve nutrient sequestration and allow for bio- film sustenance when nutrients become limited. Though the functions of cell differentiation and programmed cell death are actually at odds, they can be described as an evolutionary progressionthat allows biofilms of prokaryotes to perform and adapt as multiceewllular organisms, a behavior that emerges to be matched through quorum sensing (Webb JS, et al 2003). CHAPTER 2 Applications of Quorum sensing in biotechnology Components of bacterial QS systems form an important part of many artificial genetic circuits that control phenomena such as bistable behavior, pulse response, spatio-temporal control of gene expression, and population control (Purnick and Weiss 2009). In this section, the applications of engineered QS systems for the production of biochemicals, tissue engineering, and mixed- species fermentations are highlighted (Fig. 2). Detailed explanation ofcurrent progress in building QS-based microbial biosensors and QS-based biocontrol are given. Lastly, discussion of QS inhibition as a viable strategy for the decline of biofouling is given. Also different applications of QS in biotechnology are given. Engineered Quorum Sensing systems The promisingfield of synthetic biology seeks to generatenovel biological systems by applying the fundamental engineering principles of standardization and hierarchical abstraction to GE engineering (Purnick and Weiss 2009). This method allows designers to build and optimize compound genetic circuits that perform new functions, such as DNA-damage-induced biofilm formation and preservation of synthetic ecosystems (Balagadde et al. 2008; Kobayashi et al. 2004). Various genetic modules can be included into complex gene networks also called genetic systems or devicesusing a plug-and-play strategy (Kobayashi et al. 2004). These gene networks are then commenced into a well-characterized, steady host cell known as a chassis, which supplies the essential raw materials and support machinery. Operation of the artificial genetic device imparts new functionalities to the host and makes a microbial cell factory that is capable of performing preferred tasks. Autoinducers are very useful as input signals as they are little, diffuse freely in aqueous media, and are simply taken up through cells. As the engineered cells synthesize QS signals by themselves, they are able to watch their own cell density with modulate their activities appropriately, thereby falling the need for outerprotection(Brenner et al. 2007). Scientists have devised QS-inducible mammalian genetic circuits by mixing bacterial QS receptors with either a eukaryotic transactivation domain or with a eukaryotic transcription repressor domain (reviwed from Neddermann et al. 2003; Weber and Fussenegger 2009; Weber et al. 2005; Weber et al. 2003; Williams et al. 2004).These synthetic gene regulation systems will have functions in drug discovery, tissue engineering, and also industrial production of biochemicalsduring mammalian cell culture. Consumption of a bistable switch module gives a pointed(ON or OFF) or binary profile of aim gene expression depending on the store concentration. Engineered QS systems including bistable switches are probable to be extremely useful in industrial production of toxic gene products and in designing environmental biosensors. Scientistshave used components of the V. fischeri Quorum Sensing system to engineer spatio-temporally keeping up cell to cell communication in E. coli (Basu et al. 2004). Depending on the comparative distribution of Sender and Receiver cells in a 2-D matrix, different reporter formats such as bullseye, ellipse, oval, heart, and clover were formed. Moreexpansion of this research into programming spatial patterning in 3-D will have applications in biosensing, tissue engineering, plus fabrication of biomaterials. Quorum Sensing like cell-to-cell communication systems have also been developed by using metabolites, antibiotics, hormonesor volatile compounds to give signals to extract a cell-density dependent population-wide reactions (Bulter et al. 2004; Chen and Weiss 2005; Weber et al. 2007). The capacity to develop QS type communication systems using non-Quorum Sensing signals considerablygrows the design possibilities for genetic engineering systems. Through inserting the producing signal components in one species, and the receptor in another, scientists have engineered inter and intra-kingdom communications among bacteria, yeast, plants, and mammalian cells (Balagadde et al. 2008; Brenner et al. 2007; Weber et al. 2007). Depending on the planned synthetic communication device, relationships like predator-prey, commensalism, mutualism, amensalismand parasitism were producedamong the communicating species. Different from engineering inter-species communication, Quorum Sensing based genetic devices can control diverse features of mixed-species fermentations. Forcase, basedon QS population control circuits can be used to manage the cell densities of the contributing species (You et al. 2004). Based on QS gene-expression circuits can also be used to initiate expression of mark genes when the cell densities of contributing species reach a definite threshold (Brenner et al. 2007). At present, the majority of the engineered QS devices are built on Gram-negative AHL systems, which, as stated previously, are absolutely unreliable. Various applications of Quorum Sensing: Biosensors An interesting application of Quorum Sensing is in the engineering of whole cell microbial biosensors to distinguish pathogenic microbes present in the environment with diseased host organisms. Quorum Sensing have also been used to produce engineered bacteria capable of attacking cancer cells. It is probable to visualize the creation of new anti-cancer therapeutics by the addition of cancer-destructing elements to these microbial biosensors. Another function of QS and quorum quenching lies in the designing of transgenic plants that are able to protect themselves against general bacterial pathogens. Pathogen diagnostics and therapeutics The majority of the whole cell QS biosensors that have been explained so far recognize Gram-negative AHLs (Kumari et al. 2008; Steindler and Venturi 2007). A standard AHL biosensor contains an AHL responsive transcriptional regulator also a cognate promoter, which directs the transcription of a reporter gene. It has been recommended that QS signals only can be used as markers for the occurrence of pathogenic bacteria in clinical and environmentalsamples. Thus, QS signals should not be engaged as the only inputs for microbial biosensors. However, Quorum sensing based amplification circuits can still be used to engineer biosensing circuits to find the occurrence of pathogenic microbes in contaminated groundwater products, dairy, and meat products. Upcoming design directions willinclude the formation of ingestible whole cell biosensors by launching QS-based bio- sensing devices into GRAS organisms such as lactic acid bacteria(Konings et al. 2000). Such diagnostic biosensors would be muc h useful in identifying the existence of pathogens in the gut micro flora. So collecting these resultsbring up the exciting possibility that future QS-based microbial biosensors may possibly not only detect pathogens, but also increase a concerted reaction against them. Cancer detection The P. aeruginosa Quorum Sensing signal 3-oxo-C12- HSL reduces proliferation alsoinduceapoptosis breast cancer cell lines in human(Li et al. 2004). Biocontrol The rhizosphere is a limited region of soil that surroundings a plants roots and is affected by secretions from the root also soil microbes in the vicinity. Quorum sensing bacteria form amain component of the rhizosphere community. Scientists have also engaged quorum-quenching enzymes to decrease bacterial virulence against plants. This researchproposes that engineering the production also secretion of quorum- quenching enzymes into plants and plant-associated microbes can also serve as a crop protection plan. Though, QS systems also controlnecessary functions in useful rhizosphere bacteria, as well as biofilm formation, antibiotic production, and nitrogen fixation (Muller et al. 2009; Sanchez-Contreras et al. 2007). More research is therefore essential to understand the promising effects of quorum quenching on plant biochemical pathways. In brief, while quorum quenching is an attractive approach for biocontrol, more research isessential to demonstrate its safety and efficacy. Prevention of biofouling Biofouling is the increase of bacteria, algae;also animals like protozoans and crustaceans on surfaces that prolonged contact with water. Biofouling can happen on surfaces as assorted as pipes, tanks, ship hull, membrane bioreactors, medical or dental implants, and catheters. This unwantedgrowth of living organisms and their secretions lead to contamination, colonization, also corrosion of machine parts expose to water and reduce machine efficiency. Incorporation of Quorum Sensing inhibitors on the device surface is a possible strategy for declining P. aeruginosa biofouling of surgical implants. QS inhibition may be used to givedefense against many pathogens that rely on QS to start biofilm development. Recombinant gene expression Possibly one of the exciting areas for research in quorum sensing is the synthesis of recombinant gene products withmetabolic engineering. Quorum sensing has been used to control gene expression and cellular growth. Brief reviews by Toniatti et al. (Toniatti C, et al 2004) discusssome of the progress in control of gene expression through the perceptions of possible gene therapy applications. Pathogen/pest management Pathogen and pest (i.e. some organism whose existence in a specific environment is undesirable) management include most of the present applications of quorum-sensing technology. Inhibition of quorum signalling is theevident and, in practice, most appreciated application of quorum-sensing knowledge. CHAPTER 3 New technologies in Quorum Sensing The discovery of antibiotics early in the past century marked the beginning of active control and prevention of infectious microbial diseases. However, extensive use of antibiotics has also unavoidably resulted in the emergence of superbugs that resist conventional antibiotics. The finding that many pathogens rely on cell-to-cell communication mechanisms, known as quorum sensing, to synchronize microbial activities essential for infection and survival in the host suggests a promising disease control strategy, i.e. quenching microbial quorum sensing or in short, quorum quenching. Work over the past few years has demonstrated that quorum-quenching mechanisms are widely conserved in many prokaryotic and eukaryotic organisms. These naturally occurring quorum-quenching mechanisms appear to play important roles in microbe-microbe and pathogen-host interactions and have been used, or served as lead compounds, in developing and formulating a new generation of antimicrobials. An advance study of bacterial quorum sensing process can facilitate development of novel technologies intended at interfering with bacterial communication and virulence. The term quorum sensing explains the capability of a microorganism to recognize and response to diffusible signal molecules. Bacterial cells sense their inhabitants density by a complicated cell-to-cell communication system also triggers expression of exact genes. Quorum sensing in Seaweeds Explaining this title, the quorum sensing is wider spread among bacterial population then was previously thought, in Gram positive, Gram-negative bacterial communication. Followed by this numerous researchers have concluded that in Gram negative bacteria acyl-homoserine lactone is dependable for the cell to cell communication system. In gram-positive bacteria peptide and derivative peptide based signaling molecules appear to be the main mode of communication. Throughout high cell density the marine bacteria can produce enzymes, surfactants, toxins, antibiotics by the chemical signal communication. Marine epibiotic bacteria are also identified to produce compounds active beside drug resistant hospital pathogen by the cross species induction process. Austin described in building on assays (Billaud and Austin 1990) a screening method has been developed in which marine bacteria are confront by exposing them to terrestrial bacteria prior to assay of antimicrobial compounds. Therefore, in currentstudies it is proposed to search the abilities of seaweed epibiotic bacterial organisms to createantibacterial compounds by quorum sensing. Theseconclusions have important consequences for the discovery of new antimicrobial compounds from marine bacteria and might allow the growth of novelprocess for screening new compounds eff ective against multidrug resistant bacteria. CHAPTER 4 Conclusion Quorum Sensing plays a main role in controlling a diversity of microbial cell activities, like biofilm formation and virulence, that considerably impact human health, agriculture, marine, commercial manufacture and transport systems. As mentioned in above applications of QS there are many areas that are fully touched by QS method. Therefore, significant research efforts are needed to understanding Quorum Sensing and the growth of strategies to disrupt and influence Quorum Sensing. Our understanding of quorum-sensing mechanisms currently restricts applications for quorum sensing. Though there has been progress made in the use of quorum sensing, more understanding of quorum functionality is necessary before the control of this tool can be completely raised. However, the full-scale management of the bacterial quorum circuit in a biotechnological application yet to be an unconvinced goal. More studyand deep research is needed to uncover andthe details of QS in a diversity of microbial species, with Gram- positive bacterias and fungis. The task of QS in microbial populations, with Quorum Sensing crosstalk and signal specificity, is another significant area of research and study that will influence strategies to prevent biofilm formation and for biocontrol. Quorum sensing seems to be a distinctive example of how the exploitation of bacteria cell-to-cell communication in biotechnology can be used to significantly drive the growth and development of medicine, diagnosis tics, therapies and gene control. For sure, it will influence every part of biology, with novel research and technologies in science world.

An Analysis of Freytag’s Five Steps in Shakespeare’s “The Tempest” Essay

In this essay I will analyze William Shakespeare’s â€Å"The Tempest† using Gustav Freytag’s five phases which are: exposition, point of conflict, rising action, climax, and denouement.   I will first begin with the exposition phase.   The exposition phase includes a description of the chief protagonist and antagonist as well as a description of their conflict and setting.   I will also pinpoint the inciting moment, which is an incident that must occur for the story to happen. (www.reference.com)   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The chief protagonist of the play is Prospero, the rightful duke of Milan.   The main antagonist is his brother, Antonio, the usurping duke of Milan.   Antonio overthrew Prospero and sent him and his young daughter Miranda out to sea.   They land on an island, which will be the central setting of the play, where Prospero learns magic and frees a spirit named Ariel who is indebted in servitude to him.   Prospero uses his magic to stir up a storm and moor a ship that his brother Antonio is on.   Ariel makes sure that the occupants of the boat arrive on the island unscathed.   The inciting incident is the arrival of Antonio and the rest of his party on the island.   The main conflict is between Prospero, who wants his dukedom reinstated, and Antonio, who wants the dukedom for himself.   Next, I will describe the rising action in the play.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The rising action includes related secondary conflicts. (www.reference.com)The first of these secondary conflicts occurs between Antonio and Sebastian, the king of Naples brother, and Gonzalo, a counselor, and Alonso, the king of Naples.   These gentlemen had been traveling together on the boat, but when they come to the island Antonio and Sebastian plan to murder Alonso and Gonzalo so that they can seize the power of the crown for themselves.   Alonso believes his son, who had been traveling with them as well to be dead and Antonio and Sebastian see this as a chance to eliminate Alonso and usurp the crown.    Alonso’s son Ferdinand really isn’t dead and is busy falling in love with Prospero’s daughter while this is happening.   The last secondary conflict begins with Caliban, Prospero’s unwilling slave, and Stephano and Trinuculo, a jester and a drunken butler who were traveling with the party on the boat, meeting each other on the island and deciding to kill Prospero.   Ariel hears this and informs Prospero thus avoiding catastrophe.   Now, I will discuss the falling action, climax, and denouement.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The climax takes place in â€Å"The Tempest† when Prospero charms the party from the boat and Caliban, Stephano, and Trinuculo into immobility.   Prospero, who is joyous at the thought of his daughter’s upcoming marriage to Ferdinand, has a change of heart and releases everyone from the spell.   They are all repentant and the falling action occurs at this point with a monologue from Prospero chastising the king of Naples and Antonio for the part the played in the usurping of his dukedom. He also chastises his slave and the butler and jester for trying to kill him.   The denouement encompasses the happy marriage of Miranda and Ferdinand and the reinstatement of Prospero’s dukedom.   The play ends happily with the protagonist better of at the end than at the outset because it is a comedy rather than a tragedy.   Thus, I have analyzed â€Å"The Tempest† and have discerned Freytag’s five phases within the play. Bibliography www.reference.com

An Analysis of J.D. Salinger’s The Catcher in the Rye

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T he death of Holden’s brother, Allie, is what caused Holden to not want to grow up and to keep his childhood adolescence. He states that people live for a while but will eventually just die. Holden decides to â€Å"numb himself† to his feelings about life to the point where it seems like he is no longer truly living but is just going through the motions day by day. Going through adolescence can be a difficult time. At some times it may feel like everyone is against you. This is similar to what Holden experienced in Catcher in the Rye. He continuously tried to alienate himself from society; he suffers from a self-imposed delusion that he is different. Throughout the story, Holden realizes that growing up indicates that he should assume responsibility in a world where he thinks only superficial and insignificant issues occur (Abdulatif, 2010). Holden is caught in the transition of his life- from the state of childhood into adulthood. 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Case Profile For The Council For Human Rights - 725 Words

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People such as failed conservative Supreme Court nominee Robert Bork, the author of â€Å"Civil Liberties After 9/11,† feel that racial profiling â€Å"does not exist† and believe that â€Å"the ideaRead MoreThe Relationship between Church and State around the World Essay1400 Words   |  6 Pages Religion can be defined as a set of beliefs concerning the cause, nature, and purpose of the universe that often contains a moral code that governs the conduct of human affairs according to dictionary.com. Some countries use their religion to form some sort of government or use religion as a way to decide the laws within their land. People often believe that religion should be kept out of government while others think it may create a more effective one. Places such as the United States, partsRead MoreManaging Diversity3506 Words   |  15 PagesManaging Diversity in The Public Sector: A case Study of a Small City Council; by Mark Steger and Prof R. 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Religion Hinduism and Islam Essay - 1277 Words

Hinduism and Islam are two largely practiced religions, specifically in India and the subcontinents. Both Hinduism and Islam have unique practices and traditions, which can vary depending on the town, region or people. They share a common belief in a supreme god, that being either Brahma or Allah respectively. The traditions differ on the central texts used, as well as differences in the process of reaching the afterlife and basic beliefs. Although, Hinduism might be regarded by some as a polytheistic religion in its most basic form Hinduism is based around one deity. The Brahma is the god that all of the other gods are derived from and each god is a manifestation of the one being. There are four other main deities, aside from Brahma,†¦show more content†¦Allah is the creator of everything and is held in the highest regard for Islamic people. Although there is one god, there are a multitude of prophets who bring the word of God to the Muslim people much like the multiple de ities who come from Brahma. It is believed that God sent multiple prophets to tell of the last and most significant prophet, Muhammad. These prophets also preached the Oneness of God and would tell followers to avoid sin. Muhammad, who is believed to be a prophet of God, is considered the last prophet sent by god to the Muslim people. Muhammad is believed to have brought the word of God through the Qur’an. The Prophets’ life started in Mecca where he grew up orphaned. During his life, specifically in the month of Ramadan, he would spend time in a cave outside Mecca where he originally received revelations about the sacred text. Originally Muhammad found little success preaching God’s word but eventually gained a following of Muslim people, which would turn into the tradition it is today. Although their belief in a supreme god aligns in both Islam and Hinduism, the traditions differ on the texts used centrally in each practice. The Veda, written between 1750 B CE and 600 BCE are ancient scriptures, which are the sacred texts of Hinduism. One of the forms the Veda is the Shruti, which is â€Å"that which was heard† and the other form is the Vedir seers or Rishis which means â€Å"saw†. The Veda is split into fourShow MoreRelatedThe Religions Of Islam, Hinduism, And Buddhism1241 Words   |  5 Pages Religion and politics are quite interwoven into society both in the developed world as well as in the developing world. This paper will discuss the major religions of Islam, Christianity, Hinduism, and Buddhism and how these faiths connect to politics of nations. Islam has always been a very mixed within state and society as a whole, with no true line between church and state. Islam began in the Arabia by the Prophet Muhammad in the seventh century, and is the second largest religion in the worldRead MoreReligion : Hinduism, Islam, And Christianity1079 Words   |  5 PagesReligion is known as having a deep relationship with a supreme being or thing. Also, to believe in them with the extent of worship and to follow their practices. A few of the main religions over time have been Hinduism, Islam, and Christianity. These three religions consist of ideas and practices that may be very different, but are made up of the same general idea expressed in a new form. Although their main differences, each religion seeks one thing, and that s an afterlife. Most religions areRead MoreWorld Religion : Judaism, Hinduism, And Islam Essay1391 Words   |  6 PagesWorld Religion At first glance and hearing about, Judaism, Hinduism, Buddhism, Christianity, and Islam these five religions-look and sound a lot alike. 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The Lost Symbol Chapter 71-75 Free Essays

string(71) " a side door of the Adams Building and out into the cold winter night\." CHAPTER 71 Mal’akh stood naked in the billowing warmth of his steam shower. He felt pure again, having washed off the last remaining scent of ethanol. As the eucalyptus-infused vapors permeated his skin, he could feel his pores opening to the heat. We will write a custom essay sample on The Lost Symbol Chapter 71-75 or any similar topic only for you Order Now Then he began his ritual. First, he rubbed depilatory chemicals across his tattooed body and scalp, removing any traces of body hair. Hairless were the gods of the seven islands of Heliades. Then he massaged Abramelin oil into his softened and receptive flesh. Abramelin is the sacred oil of the great Magi. Then he turned his shower lever hard to the left, and the water turned ice cold. He stood beneath the frigid water for a full minute to close his pores and trap the heat and energy within his core. The cold served as a reminder of the icy river in which this transformation had begun. When he stepped from the shower, he was shivering, but within seconds, his core heat emanated up through his layers of flesh and warmed him. Mal’akh’s insides felt like a furnace. He stood naked before the mirror and admired his form . . . perhaps the last time he would see himself as a mere mortal. His feet were the talons of a hawk. His legs–Boaz and Jachin–were the ancient pillars of wisdom. His hips and abdomen were the archways of mystical power. Hanging beneath the archway, his massive sex organ bore the tattooed symbols of his destiny. In another life, this heavy shaft of flesh had been his source of carnal pleasure. But no longer. I have been purified. Like the mystical eunuch monks of Katharoi, Mal’akh had removed his testicles. He had sacrificed his physical potency for a more worthy one. Gods have no gender. Having shed the human imperfection of gender along with the earthly pull of sexual temptation, Mal’akh had become like Ouranos, Attis, Sporus, and the great castrati magicians of Arthurian legend. Every spiritual metamorphosis is preceded by a physical one. Such was the lesson of all the great gods . . . from Osiris, to Tammuz, to Jesus, to Shiva, to the Buddha himself. I must shed the man who clothes me. Abruptly, Mal’akh drew his gaze upward, past the double-headed phoenix on his chest, past the collage of ancient sigils adorning his face, and directly to the top of his head. He tipped his head toward the mirror, barely able to see the circle of bare flesh that waited there. This location on the body was sacred. Known as the fontanel, it was the one area of the human skull that remained open at birth. An oculus to the brain. Although this physiological portal closes within a matter of months, it remains a symbolic vestige of the lost connection between the outer and inner worlds. Mal’akh studied the sacred patch of virginal skin, which was enclosed by the crownlike circle of an ouroboros–a mystical snake devouring its own tail. The bare flesh seemed to stare back at him . . . bright with promise. Robert Langdon soon would uncover the great treasure that Mal’akh required. Once Mal’akh possessed it, the void on top of his head would be filled, and he would at last be prepared for his final transformation. Mal’akh padded across his bedroom and took from his bottom drawer a long strip of white silk. As he had done many times before, he wrapped it around his groin and buttocks. Then he went downstairs. In his office, his computer had received an e-mail message. It was from his contact: WHAT YOU REQUIRE IS NOW WITHIN REACH. I WILL CONTACT YOU WITHIN THE HOUR. PATIENCE. Mal’akh smiled. It was time to make final preparations. CHAPTER 72 The CIA field agent was in a foul mood as he descended from the reading-room balcony. Bellamy lied to us. The agent had seen no heat signatures whatsoever upstairs near the Moses statue, nor anywhere else upstairs for that matter. So where the hell did Langdon go? The agent retraced his steps now to the only place they’d spotted any heat signatures at all–the library’s distribution hub. He descended the stairs again, moving beneath the octagonal console. The noise of the rumbling conveyors was grating. Advancing into the space, he flipped down his thermal goggles and scanned the room. Nothing. He looked toward the stacks, where the mangled door still showed hot from the explosion. Other than that, he saw no– Holy shit! The agent jumped back as an unexpected luminescence drifted into his field of vision. Like a pair of ghosts, the dimly glowing imprints of two humanoids had just emerged from the wall on a conveyor belt. Heat signatures. Stunned, the agent watched as the two apparitions circled the room on the conveyor loop and then disappeared headfirst into a narrow hole in the wall. They rode the conveyor out? That’s insanity. In addition to realizing they had just lost Robert Langdon through a hole in the wall, the field agent was now aware that he had another problem. Langdon’s not alone? He was just about to switch on his transceiver and call the team leader, but the team leader beat him to it. â€Å"All points, we’ve got an abandoned Volvo on the plaza in front of the library. Registered to one Katherine Solomon. Eyewitness says she entered the library not long ago. We suspect she’s with Robert Langdon. Director Sato has ordered that we find them both immediately.† â€Å"I’ve got heat signatures for both of them!† shouted the field agent in the distribution room. He explained the situation. â€Å"For Christ’s sake!† the team leader replied. â€Å"Where the hell does the conveyor go?† The field agent was already consulting the employee reference schematic on the bulletin board. â€Å"Adams Building,† he replied. â€Å"One block from here.† â€Å"All points. Redirect to the Adams Building! NOW!† CHAPTER 73 Sanctuary. Answers. The words echoed in Langdon’s mind as he and Katherine burst through a side door of the Adams Building and out into the cold winter night. You read "The Lost Symbol Chapter 71-75" in category "Essay examples" The mysterious caller had conveyed his location cryptically, but Langdon had understood. Katherine’s reaction to their destination had been surprisingly sanguine: Where better to find One True God? Now the question was how to get there. Langdon spun in place, trying to get his bearings. It was dark, but thankfully the weather had cleared. They were standing in a small courtyard. In the distance, the Capitol Dome looked startlingly far away, and Langdon realized this was the first moment he had stepped outside since arriving at the Capitol several hours ago. So much for my lecture. â€Å"Robert, look.† Katherine pointed toward the silhouette of the Jefferson Building. Langdon’s first reaction on seeing the building was astonishment that they had traveled so far underground on a conveyor belt. His second reaction, however, was alarm. The Jefferson Building was now abuzz with activity–trucks and cars pulling in, men shouting. Is that a searchlight? Langdon grabbed Katherine’s hand. â€Å"Come on.† They ran northeast across the courtyard, quickly disappearing from view behind an elegant U- shaped building, which Langdon realized was the Folger Shakespeare Library. This particular building seemed appropriate camouflage for them tonight, as it housed the original Latin manuscript of Francis Bacon’s New Atlantis, the utopian vision on which the American forefathers had allegedly modeled a new world based on ancient knowledge. Even so, Langdon would not be stopping. We need a cab. They arrived at the corner of Third Street and East Capitol. The traffic was sparse, and Langdon felt fading hope as he scanned for taxis. He and Katherine hurried northward on Third Street, putting distance between themselves and the Library of Congress. It was not until they had gone an entire block that Langdon finally spotted a cab rounding the corner. He flagged it down, and the cab pulled over. Middle Eastern music played on his radio, and the young Arab driver gave them a friendly smile. â€Å"Where to?† the driver asked as they jumped into the car. â€Å"We need to go to–â€Å" â€Å"Northwest!† Katherine interjected, pointing up Third Street away from the Jefferson Building. â€Å"Drive toward Union Station, then left on Massachusetts Avenue. We’ll tell you when to stop.† The driver shrugged, closed the Plexiglas divider, and turned his music back on. Katherine shot Langdon an admonishing look as if to say: â€Å"Leave no trail.† She pointed out the window, directing Langdon’s attention to a black helicopter that was skimming in low, approaching the area. Shit. Sato was apparently dead serious about recovering Solomon’s pyramid. As they watched the helicopter land between the Jefferson and Adams buildings, Katherine turned to him, looking increasingly worried. â€Å"Can I see your cell phone for a second?† Langdon handed her his phone. â€Å"Peter told me you have an eidetic memory?† she said, rolling down her window. â€Å"And that you remember every phone number you’ve ever dialed?† â€Å"That’s true, but–â€Å" Katherine hurled his phone out into the night. Langdon spun in his seat and watched as his cell phone cartwheeled and splintered into pieces on the pavement behind them. â€Å"Why did you do that!† â€Å"Off the grid,† Katherine said, her eyes grave. â€Å"This pyramid is our only hope of finding my brother, and I have no intention of letting the CIA steal it from us.† In the front seat, Omar Amirana bobbed his head and hummed along with his music. Tonight had been slow, and he felt blessed to finally have a fare. His cab was just passing Stanton Park, when the familiar voice of his company dispatcher crackled over the radio. â€Å"This is Dispatch. All vehicles in the area of the National Mall. We have just received a bulletin from government authorities regarding two fugitives in the area of the Adams Building . . .† Omar listened in amazement as Dispatch described the precise couple in his cab. He stole an uneasy glance in his rearview mirror. Omar had to admit, the tall guy did look familiar somehow. Did I see him on America’s Most Wanted? Gingerly, Omar reached for his radio handset. â€Å"Dispatch?† he said, speaking quietly into the transceiver. â€Å"This is cab one-three-four. The two people you asked about–they are in my cab . . . right now.† Dispatch immediately advised Omar what to do. Omar’s hands were trembling as he called the phone number Dispatch had given him. The voice that answered was tight and efficient, like that of a soldier. â€Å"This is Agent Turner Simkins, CIA field ops. Who is this?† â€Å"Um . . . I’m the taxi driver?† Omar said. â€Å"I was told to call about the two–â€Å" â€Å"Are the fugitives currently in your vehicle? Answer only yes or no.† â€Å"Yes.† â€Å"Can they hear this conversation? Yes or no?† â€Å"No. The slider is–â€Å" â€Å"Where are you taking them?† â€Å"Northwest on Massachusetts.† â€Å"Specific destination?† â€Å"They didn’t say.† The agent hesitated. â€Å"Is the male passenger carrying a leather bag?† Omar glanced in the rearview mirror, and his eyes went wide. â€Å"Yes! That bag doesn’t have explosives or anything in–â€Å" â€Å"Listen carefully,† the agent said. â€Å"You are in no danger so long as you follow my directions exactly. Is that clear?† â€Å"Yes, sir.† â€Å"What is your name?† â€Å"Omar,† he said, breaking a sweat. â€Å"Listen, Omar,† the man said calmly. â€Å"You’re doing great. I want you to drive as slowly as possible while I get my team out in front of you. Do you understand?† â€Å"Yes, sir.† â€Å"Also, is your cab equipped with an intercom system so you can communicate with them in the backseat?† â€Å"Yes, sir.† â€Å"Good. Here’s what I want you to do.† CHAPTER 74 The Jungle, as it is known, is the centerpiece of the U.S. Botanic Garden (USBG)–America’s living museum–located adjacent to the U.S. Capitol Building. Technically a rain forest, the Jungle is housed in a towering greenhouse, complete with soaring rubber trees, strangler figs, and a canopy catwalk for more daring tourists. Normally, Warren Bellamy felt nurtured by the Jungle’s earthy smells and the sunlight glinting through the mist that filtered down from the vapor nozzles in the glass ceiling. Tonight, however, lit only by moonlight, the Jungle terrified him. He was sweating profusely, writhing against the cramps that now stabbed at his arms, still pinned painfully behind him. Director Sato paced before him, puffing calmly on her cigarette–the equivalent of ecoterrorism in this carefully calibrated environment. Her face looked almost demonic in the smoke-filled moonlight that streamed down through the glass ceiling overhead. â€Å"So then,† Sato continued, â€Å"when you arrived at the Capitol tonight, and you discovered that I was already there . . . you made a decision. Rather than making your presence known to me, you descended quietly into the SBB, where, at great risk to yourself, you attacked Chief Anderson and myself, and you helped Langdon escape with the pyramid and capstone.† She rubbed her shoulder. â€Å"An interesting choice.† A choice I would make again, Bellamy thought. â€Å"Where is Peter?† he demanded angrily. â€Å"How would I know?† Sato said. â€Å"You seem to know everything else!† Bellamy fired back at her, making no attempt to hide his suspicion that she was somehow behind all this. â€Å"You knew to go to the Capitol Building. You knew to find Robert Langdon. And you even knew to X-ray Langdon’s bag to find the capstone. Obviously, someone is giving you a lot of inside information.† Sato laughed coldly and stepped closer to him. â€Å"Mr. Bellamy, is that why you attacked me? Do you think I’m the enemy? Do you think I’m trying to steal your little pyramid?† Sato took a drag on her cigarette and blew the smoke out of her nostrils. â€Å"Listen carefully. No one understands better than I do the importance of keeping secrets. I believe, as you do, that there is certain information to which the masses should not be privy. Tonight, however, there are forces at work that I fear you have not yet grasped. The man who kidnapped Peter Solomon holds enormous power . . . a power that you apparently have yet to realize. Believe me, he is a walking time bomb . . . capable of initiating a series of events that will profoundly change the world as you know it.† â€Å"I don’t understand.† Bellamy shifted on the bench, his arms aching in his handcuffs. â€Å"You don’t need to understand. You need to obey. Right now, my only hope of averting a major disaster is to cooperate with this man . . . and to give him exactly what he wants. Which means, you are going to call Mr. Langdon and tell him to turn himself in, along with the pyramid and capstone. Once Langdon is in my custody, he will decrypt the pyramid’s inscription, obtain whatever information this man is demanding, and provide him with exactly what he wants.† The location of the spiral staircase that leads to the Ancient Mysteries? â€Å"I can’t do that. I’ve taken vows of secrecy.† Sato erupted. â€Å"I don’t give a damn what you’ve vowed, I will throw you in prison so fast–â€Å" â€Å"Threaten me all you like,† Bellamy said defiantly. â€Å"I will not help you.† Sato took a deep breath and spoke now in a fearsome whisper. â€Å"Mr. Bellamy, you have no idea what’s really going on tonight, do you?† The tense silence hung for several seconds, finally broken by the sound of Sato’s phone. She plunged her hand into her pocket and eagerly snatched it out. â€Å"Talk to me,† she answered, listening carefully to the reply. â€Å"Where is their taxi now? How long? Okay, good. Bring them to the U.S. Botanic Garden. Service entrance. And make sure you get me that god-damn pyramid and capstone.† Sato hung up and turned back to Bellamy with a smug smile. â€Å"Well then . . . it seems you’re fast outliving your usefulness.† CHAPTER 75 Robert Langdon stared blankly into space, feeling too tired to urge the slow-moving taxi driver to pick up the pace. Beside him, Katherine had fallen silent, too, looking frustrated by their lack of understanding of what made the pyramid so special. They had again been through everything they knew about the pyramid, the capstone, and the evening’s strange events; they still had no ideas as to how this pyramid could possibly be considered a map to anything at all. Jeova Sanctus Unus? The secret hides within The Order? Their mysterious contact had promised them answers if they could meet him at a specific place. A refuge in Rome, north of the Tiber. Langdon knew the forefathers’ â€Å"new Rome† had been renamed Washington early in her history, and yet vestiges of their original dream remained: the Tiber’s waters still flowed into the Potomac; senators still convened beneath a replica of St. Peter’s dome; and Vulcan and Minerva still watched over the Rotunda’s long-extinguished flame. The answers sought by Langdon and Katherine were apparently waiting for them just a few miles ahead. Northwest on Massachusetts Avenue. Their destination was indeed a refuge . . . north of Washington’s Tiber Creek. Langdon wished the driver would speed up. Abruptly, Katherine jolted upright in her seat, as if she had made a sudden realization. â€Å"Oh my God, Robert!† She turned to him, her face going white. She hesitated a moment and then spoke emphatically. â€Å"We’re going the wrong way!† â€Å"No, this is right,† Langdon countered. â€Å"It’s northwest on Massachu–â€Å" â€Å"No! I mean we’re going to the wrong place!† Langdon was mystified. He had already told Katherine how he knew what location was being described by the mysterious caller. It contains ten stones from Mount Sinai, one from heaven itself, and one with the visage of Luke’s dark father. Only one building on earth could make those claims. And that was exactly where this taxi was headed. â€Å"Katherine, I’m certain the location is correct.† â€Å"No!† she shouted. â€Å"We don’t need to go there anymore. I figured out the pyramid and capstone! I know what this is all about!† Langdon was amazed. â€Å"You understand it?† â€Å"Yes! We have to go to Freedom Plaza instead!† Now Langdon was lost. Freedom Plaza, although nearby, seemed totally irrelevant. â€Å"Jeova Sanctus Unus!† Katherine said. â€Å"The One True God of the Hebrews. The sacred symbol of the Hebrews is the Jewish star–the Seal of Solomon–an important symbol to the Masons!† She fished a dollar bill out of her pocket. â€Å"Give me your pen.† Bewildered, Langdon pulled a pen from his jacket. â€Å"Look.† She spread the bill out on her thigh and took his pen, pointing to the Great Seal on the back. â€Å"If you superimpose Solomon’s seal on the Great Seal of the United States . . .† She drew the symbol of a Jewish star precisely over the pyramid. â€Å"Look what you get!† Langdon looked down at the bill and then back at Katherine as if she were mad. â€Å"Robert, look more closely! Don’t you see what I’m pointing at?† He glanced back at the drawing. What in the world is she getting at? Langdon had seen this image before. It was popular among conspiracy theorists as â€Å"proof† that the Masons held secret influence over our early nation. When the six-pointed star was laid perfectly over the Great Seal of the United States, the star’s top vertex fit perfectly over the Masonic all-seeing eye . . . and, quite eerily, the other five vertices clearly pointed to the letters M-A-S-O-N. â€Å"Katherine, that’s just a coincidence, and I still don’t see how it has anything to do with Freedom Plaza.† â€Å"Look again!† she said, sounding almost angry now. â€Å"You’re not looking where I am pointing! Right there. Don’t you see it?† An instant later, Langdon saw it. CIA field-operations leader Turner Simkins stood outside the Adams Building and pressed his cell phone tightly to his ear, straining to hear the conversation now taking place in the back of the taxi. Something just happened. His team was about to board the modified Sikorsky UH-60 helicopter to head northwest and set up a roadblock, but now it seemed the situation had suddenly changed. Seconds ago, Katherine Solomon had begun insisting they were going to the wrong destination. Her explanation–something about the dollar bill and Jewish stars–made no sense to the team leader, nor, apparently, to Robert Langdon. At least at first. Now, however, Langdon seemed to have grasped her meaning. â€Å"My God, you’re right!† Langdon blurted. â€Å"I didn’t see it earlier!† Suddenly Simkins could hear someone banging on the driver’s divider, and then it slid open. â€Å"Change of plans,† Katherine shouted to the driver. â€Å"Take us to Freedom Plaza!† â€Å"Freedom Plaza?† the cabbie said, sounding nervous. â€Å"Not northwest on Massachusetts?† â€Å"Forget that!† Katherine shouted. â€Å"Freedom Plaza! Go left here! Here! HERE!† Agent Simkins heard the cab screeching around a corner. Katherine was talking excitedly again to Langdon, saying something about the famous bronze cast of the Great Seal embedded in the plaza. â€Å"Ma’am, just to confirm,† the cabbie’s voice interjected, sounding tense. â€Å"We’re going to Freedom Plaza–on the corner of Pennsylvania and Thirteenth?† â€Å"Yes!† Katherine said. â€Å"Hurry!† â€Å"It’s very close. Two minutes.† Simkins smiled. Nicely done, Omar. As he dashed toward the idling helicopter, he shouted to his team. â€Å"We’ve got them! Freedom Plaza! Move!† How to cite The Lost Symbol Chapter 71-75, Essay examples

Bury My Heart at Wounded Knee free essay sample

Americans who have always looked westward when reading about this period should read this book facing eastward†. Despite the popularity of the eyewitness accounts, Brown is not an absentee narrator. In the book Brown emphasizes two main points, the language he uses and the storyline of the book. He uses these two things to give the eyewitness accounts as much impact as possible. In the process, he attempts to defile his enemy in all kinds of different manners: The way Brown makes his readers; view eastward is by using the faults that have plagued the Native Americans. Brown’s way of emphasizes language allows the readers to connect to the Native Americans and this allows the book to thrive and continue. This book differs from a lot of other books about Native Americans, because he uses many Native American interpretations. For example, the Sioux and Cheyenne’s frequently see trains pass through their land in the Powder River country. We will write a custom essay sample on Bury My Heart at Wounded Knee or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Says Brown: ‘‘Sometimes they saw Iron Horses dragging wooden houses on wheels at great speed along the tracks . They were puzzled over what could be inside the houses. ’’ Brown uses the terms ‘‘Iron Horses’’ and ‘‘wooden houses’’ to describe trains and train cars, as a Native American at this time would have perceived them. Brown also uses the Native American designations for U. S. military ranks in his descriptions. For example, to a Native American at this time, a general was known as a â€Å"Star Chief† and a colonel was an â€Å"Eagle Chief†. In addition, Brown refers to prominent American historical figures by their Native American names. For example, many Native Americans called General George Armstrong Custer ‘‘Hard Backsides,† â€Å"because he chased them over long distances for many hours without leaving his saddle †. Brown also uses Native American naming systems for natural processes like time. Because Americans during this time divide the year into twelve months and refer to these months by names like May and June, however, Native Americans referred to these time periods by their relationship to nature. So, in Brown’s book, May is â€Å"the Moon When the Ponies Shed† and June is â€Å"the Strawberry Moon†. By using distinctly Native American interpretations like these in his narration, Brown takes his readers deep into the Native American experience. In the process, the reader begins to identify with the Native Americans. When readers identify with characters, they tend to feel sympathy for them. Through Dee Browns thesis, Brown organizes his story to maximize his readers’ sympathetic emotions. Brown establishes a three? part structure for most chapters, which demonstrates again and again that Native Americans lost no matter what they did. Francis Paul Prucha for examples states that â€Å"The materials have been selected to make the authors point, not to present a balanced view of what happened, from the Native American standpoint or from any other†. Typically, the chapter begins with a discussion of a chief or tribe who has lost something, generally a piece of their land and still has more to lose. For example, in the beginning of the second chapter, Brown notes: â€Å"As the result of two deceptive treaties, the woodland Sioux surrendered nine? tenths of their land and were crowded into a narrow strip of territory along the Minnesota River†. Following the discussion of what has been already lost; Brown introduces the second part, the struggle. For Native Americans in the nineteenth century, the struggles were many, whether they decided to go to war or did not. Many tribes in the book do choose to fight to retain their remaining land and freedom. In most cases, the tribes win some battles but end up losing the war. The U. S. soldiers are too advanced and numerous to be defeated, something that the Native Americans begin to realize. For example, Little Crow is cautious about fighting at first, because he had been to the East and seen the power of the Americans. They were everywhere and with cannons they would destroy everything in their path. Even when the Native Americans outnumber the whites, the military technology can be the decisive factor in the victory. As many Native Americans learned, even though they had bravery, numbers, and massive charges all of that would mean nothing if the Native Americans were armed only with bows, lances, and clubs. In cases where the Native Americans try to remain peaceful, Brown shows many ways that they are provoked into war. In several cases, settlers or miners hungry for the Native Americans’ remaining land spread lies in an effort to get the government to take their land. During the Civil War, Native Americans were sometimes provoked into fighting because it was the safer of two options for white, male citizens. For example, Brown says there was political pressure on soldiers from Coloradans who wanted to avoid the military draft of 1864 by serving in uniform against a few poorly armed Indians rather than against the Confederates farther east. Even after the Civil War, when the draft was no longer an issue, some drafted soldiers used lies to provoke Native Americans and kill them because peace was not profitable for the settlers. The final part of Brown’s argument in most chapters is the ending. Due to the massive struggles that Native Americans faced whether or not they chose to remain peaceful, most chapters end badly. The chiefs, who are often depicted as strong in the beginning and middle of the chapters when they are fighting for their land and people, end up dead, in prison, in exile, or on a reservation with the rest of their people. Even the exceptions to this rule, such as the chapter depicting Red Clouds successful war, ultimately end negatively. Red Cloud’s story is an example of the overall structure of the book. The book starts out with many Native Americans living free and retaining parcels of their land. As the story progresses and the white emigration start to take over, large armies and groups of white settlers cut down the various tribes. By the end of the book, the effect of white emigration has impacted around so much of the country that most Native Americans are dead, in prison, or on scattered reservations. To conclude I felt the effect on the reader is profound. Brown has gotten his readers to root for the underdogs by using eyewitness accounts and language to draw readers into the Native American experience. Yet, in each chapter Brown steadily crushes any hope that the reader might have for the Native Americans winning much of anything by using his plot. By using these strategies, Brown makes his readers feel more into the book by trying to make them sympathize to the Natives. Brown’s tone, or attitude towards his subject matter, is one of barely restrained outrage, and he wants readers to get angry, too. Tom Phillips, another reviewer states, â€Å"Brown has gone too far at some points and is guilty of the same faults as those who created the raditional image of the Native American as savage, alcoholic and expendable†.