Quorum Sensing And Its Importance To Biotechnology

Quorum Sensing And Its Importance To Biotechnology Quorum sensing offers potential to create engineered bacteria capable of invading cancer cells. It is possible to envision the creation of novel anti-cancer therapeutics by the addition of cancer-destructing modules to these microbial biosensors. Another application of QS and quorum quenching lies in the creation of transgenic plants that are able to defend themselves against common bacterial pathogens. It plays a main role in controlling a diversity of microbial cell activities, such as biofilm formation and virulence that considerably impact human health, agriculture, and commercial production and transport systems. Quorum sensing is cell-to-cell communication in bacteria have ability to control development, sporulation, and antibiotic synthesis also virulence factor induction, cell differentiation, moreover nutrient flux along with extra physiological events in pathogenic bacterial infections. Scientists now a days creating more possible benefits from quorum sensing and off course there is lots of potential development for advancement in 2011ranging from marine to human disorders. CHAPTER 1 INTRODUCTION Several unicellular microorganisms use smallsignaling molecules to find out their local concentration. The processes involved in the production and recognitionof these signals are generally known as quorum sensing (QS). Unicellular microorganisms to manage their activities use this kind of cell-to-cell communication, which allows them to work as multi-cellular systems. Newly, several groups have confirmed artificial intraspeciesand inter-species communication through synthetic circuits, which incorporate components of bacterial QSsystems. Engineered QS-based circuits have a broad range of applications such as production of biochemicals, tissueengineering, also mixed-species fermentations. They are also extremely useful in designing microbial biosensors toidentify bacterial species present in the environment andinside living organisms. In this articlethe different ways inwhich researchers have designed QS-based circuits andtheir applications in biotechnology are explained. A decade ago, the secretion and perception of minorsignalling molecules that in turn are transduced tocoordinate behavior of a smallest unit of microorganisms was named quorum sensing by EP Greenberg with colleagues. Ever since then, an exponential growth in understanding and occurrence of quorum-sensing systemshas developed, with sightings ranging from virulence inhuman along with plant pathogens to degradative capacity ofactivated sludge. Not amazingly, regulatory mechanisms span traditional inducer/repressor motifs homologous to thelac operon to the newly discovered interfering RNAs.Advance characterization of signalling circuits, coupled with creative position applications, propose a wealth of prospects for advancing commercial biotechnology(reviewed by John C March and William E Bentley 2004). Researchers in biotechnology continuously seek new platforms from which to address problems: manifesto that, in a broad sense, improve efficacy, while maintaining or intensifying specificity. Most freshly, microbial quorum sensing has emerged as such a technology. Because microbial communities absorb a small space, concentrations of extracellular signalling molecules build up, providing motivation for unique and various cellular responses along with protection from rival microbial communities. Referred to as quorum sensing for its regularly reported and concurrent dependence on high population density (Joyce EA et al 2004), extracellular signalinggives a novel basis for control over molecular also cellular processes along with population behaviour, possibly in a manner more reliable with that of native physiology. Quorum sensing might be the base upon which the more complicated intracellular communication found in advanced level organisms has evolved. Defining quorum sensing Quorum Sensing considered a signalling molecule, a compound has to result a reaction in a population of cells that is different from the approach in which the cells would perform independently. There are two types of quorum sensing: species- specific and interspecies. Species-specific quorum sensing in Gram-negative bacteria is intercede by acyl-homoserine lactones (AHLs) with numerous moieties distinguishing signals between species (Fuqua C, Parsek MR, Greenberg EP 2001). In Gram- positive bacteria, species-specific quorum sensing is generallyassist through small peptides Figure1.Structure of bacterial Quurom sensing signals. Gram-negative bacteria like V.Fischeri and Pseudomonas aeruginosa use acyl homoserine lactones (AHLs) as signals. The structure of mature AIP-I (from Staphylococcus aureus) is shown as a representative of the translationally derived auto-inducing peptides (AIPs) used by Gram-positive bacteria as signals for QS.Source- Functions of quorum sensing Quorum sensing is supposed to control ability development, sporulation, and antibiotic synthesis also virulence factor induction, cell differentiation, and nutrient flux along with extra physiological events in pathogenic bacterial infections (Cvitkovitch DGGreenberg EP,Yarwood JM,2003).More lately, quorum sensing was connected through proteomic analysis to increased pathogenic ability in tubercular strains of Pseudomonas aeruginosa (Arevalo-Ferro C, et all 2004) Webb and co-workers (Webb JS, et al 2003), reviewed work on programmed cell death plusmicro colony differentiation in biofilms. As biofilms age, cellular differentiation and death improve nutrient sequestration and allow for bio- film sustenance when nutrients become limited. Though the functions of cell differentiation and programmed cell death are actually at odds, they can be described as an evolutionary progressionthat allows biofilms of prokaryotes to perform and adapt as multiceewllular organisms, a behavior that emerges to be matched through quorum sensing (Webb JS, et al 2003). CHAPTER 2 Applications of Quorum sensing in biotechnology Components of bacterial QS systems form an important part of many artificial genetic circuits that control phenomena such as bistable behavior, pulse response, spatio-temporal control of gene expression, and population control (Purnick and Weiss 2009). In this section, the applications of engineered QS systems for the production of biochemicals, tissue engineering, and mixed- species fermentations are highlighted (Fig. 2). Detailed explanation ofcurrent progress in building QS-based microbial biosensors and QS-based biocontrol are given. Lastly, discussion of QS inhibition as a viable strategy for the decline of biofouling is given. Also different applications of QS in biotechnology are given. Engineered Quorum Sensing systems The promisingfield of synthetic biology seeks to generatenovel biological systems by applying the fundamental engineering principles of standardization and hierarchical abstraction to GE engineering (Purnick and Weiss 2009). This method allows designers to build and optimize compound genetic circuits that perform new functions, such as DNA-damage-induced biofilm formation and preservation of synthetic ecosystems (Balagadde et al. 2008; Kobayashi et al. 2004). Various genetic modules can be included into complex gene networks also called genetic systems or devicesusing a plug-and-play strategy (Kobayashi et al. 2004). These gene networks are then commenced into a well-characterized, steady host cell known as a chassis, which supplies the essential raw materials and support machinery. Operation of the artificial genetic device imparts new functionalities to the host and makes a microbial cell factory that is capable of performing preferred tasks. Autoinducers are very useful as input signals as they are little, diffuse freely in aqueous media, and are simply taken up through cells. As the engineered cells synthesize QS signals by themselves, they are able to watch their own cell density with modulate their activities appropriately, thereby falling the need for outerprotection(Brenner et al. 2007). Scientists have devised QS-inducible mammalian genetic circuits by mixing bacterial QS receptors with either a eukaryotic transactivation domain or with a eukaryotic transcription repressor domain (reviwed from Neddermann et al. 2003; Weber and Fussenegger 2009; Weber et al. 2005; Weber et al. 2003; Williams et al. 2004).These synthetic gene regulation systems will have functions in drug discovery, tissue engineering, and also industrial production of biochemicalsduring mammalian cell culture. Consumption of a bistable switch module gives a pointed(ON or OFF) or binary profile of aim gene expression depending on the store concentration. Engineered QS systems including bistable switches are probable to be extremely useful in industrial production of toxic gene products and in designing environmental biosensors. Scientistshave used components of the V. fischeri Quorum Sensing system to engineer spatio-temporally keeping up cell to cell communication in E. coli (Basu et al. 2004). Depending on the comparative distribution of Sender and Receiver cells in a 2-D matrix, different reporter formats such as bullseye, ellipse, oval, heart, and clover were formed. Moreexpansion of this research into programming spatial patterning in 3-D will have applications in biosensing, tissue engineering, plus fabrication of biomaterials. Quorum Sensing like cell-to-cell communication systems have also been developed by using metabolites, antibiotics, hormonesor volatile compounds to give signals to extract a cell-density dependent population-wide reactions (Bulter et al. 2004; Chen and Weiss 2005; Weber et al. 2007). The capacity to develop QS type communication systems using non-Quorum Sensing signals considerablygrows the design possibilities for genetic engineering systems. Through inserting the producing signal components in one species, and the receptor in another, scientists have engineered inter and intra-kingdom communications among bacteria, yeast, plants, and mammalian cells (Balagadde et al. 2008; Brenner et al. 2007; Weber et al. 2007). Depending on the planned synthetic communication device, relationships like predator-prey, commensalism, mutualism, amensalismand parasitism were producedamong the communicating species. Different from engineering inter-species communication, Quorum Sensing based genetic devices can control diverse features of mixed-species fermentations. Forcase, basedon QS population control circuits can be used to manage the cell densities of the contributing species (You et al. 2004). Based on QS gene-expression circuits can also be used to initiate expression of mark genes when the cell densities of contributing species reach a definite threshold (Brenner et al. 2007). At present, the majority of the engineered QS devices are built on Gram-negative AHL systems, which, as stated previously, are absolutely unreliable. Various applications of Quorum Sensing: Biosensors An interesting application of Quorum Sensing is in the engineering of whole cell microbial biosensors to distinguish pathogenic microbes present in the environment with diseased host organisms. Quorum Sensing have also been used to produce engineered bacteria capable of attacking cancer cells. It is probable to visualize the creation of new anti-cancer therapeutics by the addition of cancer-destructing elements to these microbial biosensors. Another function of QS and quorum quenching lies in the designing of transgenic plants that are able to protect themselves against general bacterial pathogens. Pathogen diagnostics and therapeutics The majority of the whole cell QS biosensors that have been explained so far recognize Gram-negative AHLs (Kumari et al. 2008; Steindler and Venturi 2007). A standard AHL biosensor contains an AHL responsive transcriptional regulator also a cognate promoter, which directs the transcription of a reporter gene. It has been recommended that QS signals only can be used as markers for the occurrence of pathogenic bacteria in clinical and environmentalsamples. Thus, QS signals should not be engaged as the only inputs for microbial biosensors. However, Quorum sensing based amplification circuits can still be used to engineer biosensing circuits to find the occurrence of pathogenic microbes in contaminated groundwater products, dairy, and meat products. Upcoming design directions willinclude the formation of ingestible whole cell biosensors by launching QS-based bio- sensing devices into GRAS organisms such as lactic acid bacteria(Konings et al. 2000). Such diagnostic biosensors would be muc h useful in identifying the existence of pathogens in the gut micro flora. So collecting these resultsbring up the exciting possibility that future QS-based microbial biosensors may possibly not only detect pathogens, but also increase a concerted reaction against them. Cancer detection The P. aeruginosa Quorum Sensing signal 3-oxo-C12- HSL reduces proliferation alsoinduceapoptosis breast cancer cell lines in human(Li et al. 2004). Biocontrol The rhizosphere is a limited region of soil that surroundings a plants roots and is affected by secretions from the root also soil microbes in the vicinity. Quorum sensing bacteria form amain component of the rhizosphere community. Scientists have also engaged quorum-quenching enzymes to decrease bacterial virulence against plants. This researchproposes that engineering the production also secretion of quorum- quenching enzymes into plants and plant-associated microbes can also serve as a crop protection plan. Though, QS systems also controlnecessary functions in useful rhizosphere bacteria, as well as biofilm formation, antibiotic production, and nitrogen fixation (Muller et al. 2009; Sanchez-Contreras et al. 2007). More research is therefore essential to understand the promising effects of quorum quenching on plant biochemical pathways. In brief, while quorum quenching is an attractive approach for biocontrol, more research isessential to demonstrate its safety and efficacy. Prevention of biofouling Biofouling is the increase of bacteria, algae;also animals like protozoans and crustaceans on surfaces that prolonged contact with water. Biofouling can happen on surfaces as assorted as pipes, tanks, ship hull, membrane bioreactors, medical or dental implants, and catheters. This unwantedgrowth of living organisms and their secretions lead to contamination, colonization, also corrosion of machine parts expose to water and reduce machine efficiency. Incorporation of Quorum Sensing inhibitors on the device surface is a possible strategy for declining P. aeruginosa biofouling of surgical implants. QS inhibition may be used to givedefense against many pathogens that rely on QS to start biofilm development. Recombinant gene expression Possibly one of the exciting areas for research in quorum sensing is the synthesis of recombinant gene products withmetabolic engineering. Quorum sensing has been used to control gene expression and cellular growth. Brief reviews by Toniatti et al. (Toniatti C, et al 2004) discusssome of the progress in control of gene expression through the perceptions of possible gene therapy applications. Pathogen/pest management Pathogen and pest (i.e. some organism whose existence in a specific environment is undesirable) management include most of the present applications of quorum-sensing technology. Inhibition of quorum signalling is theevident and, in practice, most appreciated application of quorum-sensing knowledge. CHAPTER 3 New technologies in Quorum Sensing The discovery of antibiotics early in the past century marked the beginning of active control and prevention of infectious microbial diseases. However, extensive use of antibiotics has also unavoidably resulted in the emergence of superbugs that resist conventional antibiotics. The finding that many pathogens rely on cell-to-cell communication mechanisms, known as quorum sensing, to synchronize microbial activities essential for infection and survival in the host suggests a promising disease control strategy, i.e. quenching microbial quorum sensing or in short, quorum quenching. Work over the past few years has demonstrated that quorum-quenching mechanisms are widely conserved in many prokaryotic and eukaryotic organisms. These naturally occurring quorum-quenching mechanisms appear to play important roles in microbe-microbe and pathogen-host interactions and have been used, or served as lead compounds, in developing and formulating a new generation of antimicrobials. An advance study of bacterial quorum sensing process can facilitate development of novel technologies intended at interfering with bacterial communication and virulence. The term quorum sensing explains the capability of a microorganism to recognize and response to diffusible signal molecules. Bacterial cells sense their inhabitants density by a complicated cell-to-cell communication system also triggers expression of exact genes. Quorum sensing in Seaweeds Explaining this title, the quorum sensing is wider spread among bacterial population then was previously thought, in Gram positive, Gram-negative bacterial communication. Followed by this numerous researchers have concluded that in Gram negative bacteria acyl-homoserine lactone is dependable for the cell to cell communication system. In gram-positive bacteria peptide and derivative peptide based signaling molecules appear to be the main mode of communication. Throughout high cell density the marine bacteria can produce enzymes, surfactants, toxins, antibiotics by the chemical signal communication. Marine epibiotic bacteria are also identified to produce compounds active beside drug resistant hospital pathogen by the cross species induction process. Austin described in building on assays (Billaud and Austin 1990) a screening method has been developed in which marine bacteria are confront by exposing them to terrestrial bacteria prior to assay of antimicrobial compounds. Therefore, in currentstudies it is proposed to search the abilities of seaweed epibiotic bacterial organisms to createantibacterial compounds by quorum sensing. Theseconclusions have important consequences for the discovery of new antimicrobial compounds from marine bacteria and might allow the growth of novelprocess for screening new compounds eff ective against multidrug resistant bacteria. CHAPTER 4 Conclusion Quorum Sensing plays a main role in controlling a diversity of microbial cell activities, like biofilm formation and virulence, that considerably impact human health, agriculture, marine, commercial manufacture and transport systems. As mentioned in above applications of QS there are many areas that are fully touched by QS method. Therefore, significant research efforts are needed to understanding Quorum Sensing and the growth of strategies to disrupt and influence Quorum Sensing. Our understanding of quorum-sensing mechanisms currently restricts applications for quorum sensing. Though there has been progress made in the use of quorum sensing, more understanding of quorum functionality is necessary before the control of this tool can be completely raised. However, the full-scale management of the bacterial quorum circuit in a biotechnological application yet to be an unconvinced goal. More studyand deep research is needed to uncover andthe details of QS in a diversity of microbial species, with Gram- positive bacterias and fungis. The task of QS in microbial populations, with Quorum Sensing crosstalk and signal specificity, is another significant area of research and study that will influence strategies to prevent biofilm formation and for biocontrol. Quorum sensing seems to be a distinctive example of how the exploitation of bacteria cell-to-cell communication in biotechnology can be used to significantly drive the growth and development of medicine, diagnosis tics, therapies and gene control. For sure, it will influence every part of biology, with novel research and technologies in science world.

An Analysis of Freytag’s Five Steps in Shakespeare’s “The Tempest” Essay

In this essay I will analyze William Shakespeare’s â€Å"The Tempest† using Gustav Freytag’s five phases which are: exposition, point of conflict, rising action, climax, and denouement.   I will first begin with the exposition phase.   The exposition phase includes a description of the chief protagonist and antagonist as well as a description of their conflict and setting.   I will also pinpoint the inciting moment, which is an incident that must occur for the story to happen. (www.reference.com)   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The chief protagonist of the play is Prospero, the rightful duke of Milan.   The main antagonist is his brother, Antonio, the usurping duke of Milan.   Antonio overthrew Prospero and sent him and his young daughter Miranda out to sea.   They land on an island, which will be the central setting of the play, where Prospero learns magic and frees a spirit named Ariel who is indebted in servitude to him.   Prospero uses his magic to stir up a storm and moor a ship that his brother Antonio is on.   Ariel makes sure that the occupants of the boat arrive on the island unscathed.   The inciting incident is the arrival of Antonio and the rest of his party on the island.   The main conflict is between Prospero, who wants his dukedom reinstated, and Antonio, who wants the dukedom for himself.   Next, I will describe the rising action in the play.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The rising action includes related secondary conflicts. (www.reference.com)The first of these secondary conflicts occurs between Antonio and Sebastian, the king of Naples brother, and Gonzalo, a counselor, and Alonso, the king of Naples.   These gentlemen had been traveling together on the boat, but when they come to the island Antonio and Sebastian plan to murder Alonso and Gonzalo so that they can seize the power of the crown for themselves.   Alonso believes his son, who had been traveling with them as well to be dead and Antonio and Sebastian see this as a chance to eliminate Alonso and usurp the crown.    Alonso’s son Ferdinand really isn’t dead and is busy falling in love with Prospero’s daughter while this is happening.   The last secondary conflict begins with Caliban, Prospero’s unwilling slave, and Stephano and Trinuculo, a jester and a drunken butler who were traveling with the party on the boat, meeting each other on the island and deciding to kill Prospero.   Ariel hears this and informs Prospero thus avoiding catastrophe.   Now, I will discuss the falling action, climax, and denouement.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The climax takes place in â€Å"The Tempest† when Prospero charms the party from the boat and Caliban, Stephano, and Trinuculo into immobility.   Prospero, who is joyous at the thought of his daughter’s upcoming marriage to Ferdinand, has a change of heart and releases everyone from the spell.   They are all repentant and the falling action occurs at this point with a monologue from Prospero chastising the king of Naples and Antonio for the part the played in the usurping of his dukedom. He also chastises his slave and the butler and jester for trying to kill him.   The denouement encompasses the happy marriage of Miranda and Ferdinand and the reinstatement of Prospero’s dukedom.   The play ends happily with the protagonist better of at the end than at the outset because it is a comedy rather than a tragedy.   Thus, I have analyzed â€Å"The Tempest† and have discerned Freytag’s five phases within the play. Bibliography www.reference.com

An Analysis of J.D. Salinger’s The Catcher in the Rye

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T he death of Holden’s brother, Allie, is what caused Holden to not want to grow up and to keep his childhood adolescence. He states that people live for a while but will eventually just die. Holden decides to â€Å"numb himself† to his feelings about life to the point where it seems like he is no longer truly living but is just going through the motions day by day. Going through adolescence can be a difficult time. At some times it may feel like everyone is against you. This is similar to what Holden experienced in Catcher in the Rye. He continuously tried to alienate himself from society; he suffers from a self-imposed delusion that he is different. Throughout the story, Holden realizes that growing up indicates that he should assume responsibility in a world where he thinks only superficial and insignificant issues occur (Abdulatif, 2010). Holden is caught in the transition of his life- from the state of childhood into adulthood. 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Case Profile For The Council For Human Rights - 725 Words

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People such as failed conservative Supreme Court nominee Robert Bork, the author of â€Å"Civil Liberties After 9/11,† feel that racial profiling â€Å"does not exist† and believe that â€Å"the ideaRead MoreThe Relationship between Church and State around the World Essay1400 Words   |  6 Pages Religion can be defined as a set of beliefs concerning the cause, nature, and purpose of the universe that often contains a moral code that governs the conduct of human affairs according to dictionary.com. Some countries use their religion to form some sort of government or use religion as a way to decide the laws within their land. People often believe that religion should be kept out of government while others think it may create a more effective one. Places such as the United States, partsRead MoreManaging Diversity3506 Words   |  15 PagesManaging Diversity in The Public Sector: A case Study of a Small City Council; by Mark Steger and Prof R. 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